Showing 3 results for Trace Elements
Abdolrasoul Hakim Elahi, Rasoul Sharifi, Minoo Mahmoodi, Seyed Mehrdad Kassaee,
Volume 14, Issue 5 (9-2020)
Abstract
Background and objectives: The aim of this study was to evaluate the effectiveness of octopine (phytogenic-derivative of arginine) on antioxidant indices, trace elements and lipid profiles of a mouse model of breast cancer.
Methods: In this study, 48 Balb/c mice were divided into six groups: healthy control, cancer control, cancer group receiving 50 mg of octopine, cancer group receiving 100 mg of octopine and cancer group receiving 150 mg of octopine. The octopine treatment was carried out for three weeks. The 4T1 cell line was used to induce cancer. Fasting blood samples were taken from mice to evaluate lipid profile, copper and zinc levels. Malondialdehyde, superoxide dismutase and glutathione peroxidase activity in breast tumor tissues was evaluated. Data were analyzed by SPSS 18 software using one-way ANOVA and t-test.
Results: Octopine had no significant effect on superoxide dismutase and glutathione peroxidase activity in the treatment group compared with the control cancer group. However, it significantly increased total antioxidant capacity and decreased malondialdehyde activities. Furthermore, treatment with octopine significantly decreased serum zinc, copper, TG, cholesterol and low-density lipoprotein levels but significantly increased high-density lipoprotein compared with the untreated cancer group.
Conclusion: Octopine administration is effective in reducing some oxidative stress indices and improving trace elements abnormalities and lipid profile in mouse models of breast cancer.
Fereshteh Hematyar Tabatabaie , Ali Asghar Moshtaghie, Ali Asghar Rastegari, Hashem Nayeri,
Volume 18, Issue 2 (3-2024)
Abstract
Background: Certain trace elements, like cerium, have the potential to disrupt iron metabolism. This study explored the impact of cerium on intestinal iron absorption, focusing on the initial stage of iron metabolism. We employed the rat everted gut sac (EGS) segments to assess the interference caused by cerium. The primary objectives of this study were to examine the absorption of cerium in the intestines and to compare iron absorption in the presence and absence of cerium.
Methods: For the EGS experiment, segments of the rat's duodenum, ileum, or jejunum were promptly excised, cut into 5-6 cm segments, and rinsed with a physiological solution. These freshly prepared rat EGS segments were then incubated in Earle's medium containing iron (III) and/or cerium (III). We examined the impact of ascorbic acid, glucose, and different time intervals on the intestinal absorption of cerium and iron. Specifically, we investigated how glucose (5 mM) and ascorbic acid (2.8 mM) affected the absorption of cerium and iron at various concentrations (ranging from 0 to 200 mg/L). Additionally, we assessed the interfering effect of cerium on iron absorption.
Results: The results indicated that the maximum intestinal absorption of Fe (III) and Ce (III) occurred at a concentration of 200 mg/L. Furthermore, it was observed that their uptake increased following the reduction by ascorbic acid. The absorption of these elements also rose in the presence of glucose, suggesting energy-dependent transport. Additionally, a consistent cerium concentration was found to decrease iron absorption by 24.3% (P ≤ 0.05).
Conclusion: Based on the results, cerium likely reduces iron uptake by competing with iron. Cerium can also disrupt iron metabolism and lead to iron-related metabolic disorders. However, further studies at the molecular and intracellular levels are needed to gain a better understanding of this mechanism.
Mahrooyeh Haji-Abolhasan Varaminfard, Mina Ebrahimi-Rad , Seifoddin Javadian , Mahmood Beheshti Monfared , Seyedeh Fatemeh Sadati Khalili, Reza Saghiri,
Volume 20, Issue 2 (3-2026)
Abstract
Background: Although several clinical and laboratory diagnostic approaches have been developed for coronary artery disease (CAD), rates of mortality and morbidity remain high, possibly because of limitations in existing diagnostic markers. Here, we evaluated the serum levels of selected trace elements (Zn, Se, and Cu) in CAD patients and healthy subjects and assessed their diagnostic values in CAD.
Methods: 53 cardiologist-approved CAD patients and 48 age- and sex-matched healthy controls were recruited. Serum levels of Zn, Se, and Cu were assessed using the atomic absorption method. GraphPad v.8.4 and SPSS v.18 software were used for statistical analyses.
Results: Serum levels of Zn and Se were significantly lower in CAD patients than in controls (P = 0.0008 and P < 0.0001, respectively). In contrast, CAD patients showed significantly higher Cu levels (P = 0.0064). Regarding the ROC curve analyses, the area under the curve (AUC) for Zn was 0.6563 (P = 0.0069). Setting the cut-off value at 1.255 μg/mL yielded a sensitivity of 56.60%, specificity of 66.67%, and likelihood ratio (LR) of 1.698. The AUC for Se was 0.8595 (P<0.0001). The optimum cut-off value of 86.50 ng/ml yielded a sensitivity of 83.02%, specificity of 75.00%, and LR of 3.321. The AUC for Cu was 0.6557 (P = 0.0071). The optimum cut-off value of 1.225 μg/mL yielded a sensitivity of 62.26%, specificity of 64.58%, and LR of 1.758.
Conclusion: Our study showed that selenium may be a biomarker with reliable diagnostic value for CAD.
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