Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
2538-4449
10.66224/mlj
en
jalali
1397
2
1
gregorian
2018
5
1
12
3
online
1
fulltext
fa
Prioritization of rs187728237 and rs80320514 as miRNA-related Variants of Human AEG-1 Gene
تحقيقي
Original Paper
<div><strong>ABSTRACT</strong><br>
<strong> Background and objectives:</strong> 3' untranslated region (3'UTR) single nucleotide polymorphisms (SNPs) represent genetic variations that may potentially affect binding of miRNA to coding genes, potentially leading to complex disorders. We aimed to perform in silico analysis of the potential phenotypic effect of 3'UTR SNPs on human <em>astrocyte elevated gene-1</em> (<em>AEG-1</em>), a newly identified candidate cancer gene.<br>
<strong> Methods:</strong> We gathered a list of all 3'UTR SNPs located in the human <em>AEG-1</em> gene from the SNP database. Analysis of the potential effects was done using MirSNP and MicroSNiper.<br>
<strong> Results:</strong> Analysis by the MirSNP estimated that rs187728237 might increase the affinity of two miRNAs and decrease the affinity of 10 other miRNAs to the AEG-1 transcript. Moreover, MicroSNiPer showed that rs80320514 might affect 24 putative miRNA binding sites in the 3'UTR of <em>AEG-1</em>.<br>
<strong> Conclusion:</strong> Based on our findings, it can be concluded that the 3'UTR SNPs located in the human <em>AEG-1</em> gene may be within the miRNA targets of the transcript, therefore affecting the stability of putative miRNA-target interactions.<br>
<strong> Keywords:</strong> AEG-1, miRNA, SNPs, 3' Untranslated Region.</div>
AEG-1, miRNA, SNPs, 3' Untranslated Region
6
11
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-518&slc_lang=fa&sid=1
Semira
Kheiri
100319475328460014422
100319475328460014422
No
Laboratory Sciences Research Center, Golestan University of Medical Science, Gorgan, Iran
Azadeh
Aliarab
100319475328460014423
100319475328460014423
No
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Hamid
Haghighatfard
100319475328460014424
100319475328460014424
No
Cellular & Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Hossein
Sadeghi
Hsadeqi86@gmail.com
100319475328460014425
100319475328460014425
Yes
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Medical Cellular & Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran