en بهداشت Health تحقیقی Original article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> <span lang="EN">Recently, the role of sports training and medicinal plants in the expression of genes has been considered to prevent the progression of diseases. The purpose of this research was to investigate the role of weight training and oleander extract on </span>interferon regulatory factor 8 (<span lang="EN">IRF8</span>) and <span lang="EN">cathepsin S (CTSS) gene expression in the soleus muscle of Alzheimer&#39;s disease (AD) model mice.</span><b><span style="font-size:8.0pt"></span></b><br> <b>Methods:</b> <span lang="EN">Fifty-five male Wistar rats were randomly divided into 5 groups: healthy control group, AD control group, AD resistance training group, AD group with the supplement of </span><i>Melilotus officinalis,</i> <span lang="EN">and AD resistance training group + </span><i>Melilotus officinalis</i> <span lang="EN">supplement. Trimethyltin-induced AD was induced. In the resistance training protocol, a weight was attached to the tail of the rats, and they had to lift this weight on a ladder with 26 steps. </span><i>Melilotus officinalis</i> <span lang="EN">was injected intraperitoneally as a supplement for 6 weeks with a dose of 300 mmol/kg. Seventy-two hours after the last training session, the rats were anesthetized, and the hippocampal tissue was immediately extracted, frozen, and analyzed. A two-way analysis of variance was used to estimate the differences between groups in control and experimental AD mice.</span><b><span style="font-size:8.0pt"></span></b><br> <b>Results:</b> <span lang="EN">There was a significant increase in the expression level of interferon-regulating factor 8 and cathepsin S genes in the AD group compared to the control group. The results of Bonferroni&#39;s post-hoc test showed that in the AD group + resistance training + <i>Melilotus officinalis</i>, a significant decrease was observed compared to the AD group (P&le;0.05).</span><b><span style="font-size:8.0pt"></span></b><br> <b>Conclusion</b>: <span lang="EN">Resistance training and the <i>Melilotus officinalis</i> extract with antioxidant mechanisms can affect CTSS and IRF8 gene expression.</span><span style="font-size:8.0pt"></span></span></span><br> &nbsp;</div> Melilotus officinalis,Resistance training,Interferon regulatory factor-8,Cathepsin S,Alzheimer disease 14 18 http://goums.ac.ir/jorjanijournal/browse.php?a_code=A-10-787-7&slc_lang=en&sid=1 farah nameni dastavard96@gmail.com 100319475328460010739 0000000198401336 Yes Department of Sport Physiology, Varamin Pishva Branch, Islamic Azad University, Varamin, Iran Sviz khalili Shavarini savizkhalili@yahoo.com 100319475328460010740 0009000490828027 No Varamin Pishva Branch, Islamic Azad University, Varamin, Iran